Freeline Therapeutics Ltd. is a privately-held clinical-stage biotechnology company based in the UK and in Germany. Our vision is to create better lives for people suffering from chronic systemic diseases using the potential of gene therapy as a one-off curative treatment. Freeline has a clear focus on AAV based gene therapy targeting the liver with the aim to provide treatments for diseases with significant unmet needs.
Our gene therapy treatment builds upon the pioneering work by the Freeline CSO, Professor Amit Nathwani, Professor of Haematology at UCL which has already transformed the lives of patients by providing safe, effective and reliable gene therapy for people with Haemophilia B.
Our proprietary AAV capsid is optimised to effectively transduce human liver cells and thus provide sustainable, high levels of the desired protein. This strong capsid performance is being validated in the haemophilia B patients and sets the stage for potentially curative products in a wide range of disease which have not to date been treatable with one-time treatment.
We are seeking a highly motivated, enthusiastic and skilled individual with an excellent track record in the laboratory, to join the Capsid Development Program to contribute to the discovery of our next AAV-based gene-therapy medicines capable of changing the treatment paradigm. The research group at Freeline places a strong emphasis on innovation, flexibility, ability to make a difference to the patients, as well as the ability to apply your skills and develop new ones by collaborating with a multidisciplinary team.
Role and Responsibilities
Under the direction of project lead design and execute various experiments to enable discovery and characterization of novel AAV capsids.
Maintain accurate records and keep databases up to date within the capsid development program, and wider Research group.
Participate in training more junior staff, help ensure the lab is a safe working environment, contribute where required to the wider R&D efforts.
Participate in drafting Laboratory Protocols/SOPs as required
Design of experiments and planning of research with support from team leader/project manager
Follow laboratory procedures, documentation and analysis of data and compilation of records in accordance with company policy
To ensure experimental validity and integrity of data at all times in accordance with company policy and/or as proscribed by more senior staff
To maintain accurate and complete records of all experiments and findings
To participate, and take a leading role, in duties associated with the effective running of the laboratory by undertaking the appropriate H&S training, maintain a high level of laboratory cleanliness and participate in any laboratory duties and rotas as required
Qualification, Education and Experience Requirements
Minimum, Degree in Biological Sciences, ideally MSc, in a relevant discipline plus relevant work experience
Significant experience in molecular biology techniques including cloning (standard RE digest, Gibson Assembly, site-directed mutagenesis), PCR, RNA/DNA purification, plasmid preparation
Experience in analytical techniques such as QPCR, RT-PCR, FACS analysis, SDS-PAGE and Western blotting
Experience in mammalian cell culture
Experience in production on adeno-associated viral vectors would be desirable.
Ability to learn new protocols, identify new approaches and techniques, and work independently as well as in multidisciplinary teams.
Ability to independently troubleshoot experimental technical issues
Ability to form a solid understanding of the global direction and design of a given project
Excellent organisational and communication skills
Ability to work to priorities and deliver projects on time
Ability to keep up-to-date detailed records of experimental work